organotypic tissue culture investigation of homocysteine thiolactone cardiotoxic effect
نویسندگان
چکیده
homocysteine thiolactone was demonstrated to inhibit the growth of 10–12-day-old chicken embryo cardiac tissue explants at 7 × 10−9 −1 × 10−3 m concentrations in a dose-dependent manner. the maximal cardiotoxic effect of homocysteine thiolactone was detected at 1 × 10−3 m, which corresponds to severe hyperhomocysteinemia. the results of experiments on culturing of cardiac tissue explants in the medium containing homocysteine thiolactone (1 × 10−3 m) and ouabain at concentrations regulating the signal-transducing (1 × 10−10 m) and pumping (1 × 10−8 m) functions of na+,k+ -atpase indicate that the cardiotoxic effect of homocysteine thiolactone is supposed to result from inhibition of the na+,k+ -atpase pumping function.
منابع مشابه
Urinary excretion of homocysteine-thiolactone in humans.
BACKGROUND A metabolite of homocysteine (Hcy), the thioester Hcy-thiolactone, has been implicated in coronary heart disease in humans. Because inadvertent reactions of Hcy-thiolactone with proteins can lead to cell and tissue damage, the ability to detoxify or eliminate Hcy-thiolactone is essential for biological integrity. We examined the hypothesis that the human body eliminates Hcy-thiolacto...
متن کاملHomocysteine thiolactone affects protein ubiquitination in yeast.
The formation of homocysteine thiolactone (HcyTl) from homocysteine occurs in all examined so far organisms including bacteria, yeast, and humans. Protein N-homocysteinylation at the ε-amino group of lysine is an adverse result of HcyTl accumulation. Since tagging of proteins by ubiquitination before their proteasomal degradation takes place at the same residue, we wondered how N-homocysteinyla...
متن کاملMethod for organotypic tissue culture in the aged animal
Organotypic slicing of brain tissue from young rodents has been used as a powerful model system for biomedical research [1], [2], [3]. Organotypic slicing complements cell culture and in vivo studies in multiple facets. This system can be useful for investigating manipulation of cellular signaling pathways without the hindrance of the blood-brain barrier while sacrificing fewer animals in the p...
متن کاملThe preparation of [35S]homocysteine thiolactone free of [35S]methionine.
The unambiguous study of homocysteine metabolism requires a source of [355]homocysteine completely free from contaminating [35S]methionine. Until now, such a reagent has not been readily available. Homocysteine is an important metabolite involved in methionine, cysteine and methylation metabolism. Homocysteine can be methylated to form methionine with either 5-methyltetrahydrofolate [1] or beta...
متن کاملThe pathophysiological hypothesis of homocysteine thiolactone-mediated vascular disease.
Accumulating evidence suggests that homocysteine (Hcy) metabolite, the thioester Hcy-thiolactone, plays an important role in atherothrombosis. Hcy-thiolactone is a product of an error-editing reaction in protein biosynthesis which forms when Hcy is mistakenly selected by methionyl-tRNA synthetase. The thioester chemistry of Hcy-thiolactone underlies its ability to from isopeptide bonds with pro...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
acta physiologica hungaricaناشر: akadémiai kiadó
ISSN 1245
دوره 102
شماره 2 2015
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023